

ORIGINAL ARTICLE 

Year : 2015  Volume
: 3
 Issue : 1  Page : 1013 

Testosterone and secondtofourth digit ratio: Their relationships in adolescent males and females of Igbo Ethnic group in Nigeria
Chikezie Jude Okamkpa^{1}, Barnabas Danborno^{1}, Shehu A Akuyam^{2}
^{1} Department of Anatomy, Ahmadu Bello University, Zaria, Nigeria ^{2} Department of Chemical Pathology, Ahmadu Bello University, Zaria, Nigeria
Date of Web Publication  13Jul2015 
Correspondence Address: Chikezie Jude Okamkpa Department of Anatomy, Faculty of Medicine, Ahmadu Bello University, Zaria Nigeria
Source of Support: None, Conflict of Interest: None  Check 
DOI: 10.4103/23157992.160733
Background: The ratio of the secondtofourth finger lengths (2D:4D) demonstrates a reliable sexual dimorphism across human ethnic groups. It may be established in utero and is negatively correlated with sperm counts and testosterone in men and positively correlated with estrogen in men and women. In view of its clinical importance, this study was carried out to document values of the 2 ^{nd} to 4 ^{th} digit ratio in the Igbo ethnic group of Nigerian population and to investigate its relationship with testosterone. Materials and Methods: A sample of 412 subjects (209 boys and 203 girls) between ages 12 and 20 years were recruited randomly for this study. Finger lengths were measured twice, using an electronic Vernier calliper (precision 0.01 mm). Blood samples were collected and analyzed for testosterone. Results: Men had lower 2D:4D ratio (0.98 ± 0.01 for the right and left hand) than women (0.99 ± 0.01 for both hands). These differences in 2D:4D between sexes were statistically significant (P < 0.001 for both hands). The other digit ratios were also lower in men than women for both hands. These differences were statistically significant for all digit ratios except for the 3D:5D and 3D:4D ratio of the both hands. Conclusion: Testosterone had an inverse relationship with 2D: 4D of both hands in males, as well as females' left hands. This result confirms that digit ratios are sexually dimorphic and useful markers of disease predisposition. Keywords: Adolescent, 2D:4D, 4D:5D, digit ratio, serum testosterone, sexual dimorphism
How to cite this article: Okamkpa CJ, Danborno B, Akuyam SA. Testosterone and secondtofourth digit ratio: Their relationships in adolescent males and females of Igbo Ethnic group in Nigeria. Ann Bioanthropol 2015;3:103 
How to cite this URL: Okamkpa CJ, Danborno B, Akuyam SA. Testosterone and secondtofourth digit ratio: Their relationships in adolescent males and females of Igbo Ethnic group in Nigeria. Ann Bioanthropol [serial online] 2015 [cited 2019 Jan 21];3:103. Available from: http://www.bioanthrojournal.org/text.asp?2015/3/1/10/160733 
Introduction   
The ratio between relative length of the index finger (2 ^{nd} digit) and ring finger (4 ^{th} digit), that is the secondtofourth digit ratio (2D:4D), is sexually dimorphic. Digit ratio has been reported by many workers to show sexual dimorphism with women having higher ratio than men. ^{[1],[2],[3],[4],[5]} Results from previous studies suggest that 2D:4D is influenced by prenatal oestrogen and testosterone levels in utero and it may predict disease predisposition. ^{[3],[6],[7]}
From finger length measurements, males have on average longer 4 ^{th} digits in relation to their 2 ^{nd} digits while females have relatively longer 2 ^{nd} digits, although their 2 ^{nd} digits are not necessarily longer than their 4 ^{th} digits. ^{[2],[8]} These sex differences in 2D:4D are reliably consistent across a number of ethnic groups and races. ^{[2],[9],[10]}
There is evidence that the 2D:4D ratio is a marker for testosterone and estrogen levels towards the end of the first trimester of pregnancy ^{[2],[3]} and reflects the action of Hox genes on differentiation during early pregnancy. ^{[11]} Today digit ratios are related to a variety of variables such as fertility, human behavior, ^{[2]} body composition, ^{[12]} predictor of birth weight, ^{[13]} aggression and assertiveness, ^{[4],[8],[14]} personality disorders, ^{[2]} gender inequalities, ^{[15]} and congenital adrenal hyperplasia. ^{[16]}
Several studies have investigated the 2D:4D ratios in many ethnic groups but studies on the other digits ratios are rather limited. To our knowledge, there are few studies on 2D:4D and other digit ratios among the Igbo ethnic group in Nigeria. In view of the clinical interest that 2D:4D and other finger ratios may have as markers of disease predisposition, and as markers of genetic or environmental influence, we investigated the 2D:4D and other digit ratios in a sample of adolescents from the Igbo ethnic group in Nigeria.
Materials and methods   
A sample of 412 adolescents from the Igbo ethnic group of Nigeria (females n = 203 and males n = 209) aged 1220 years, selected by random procedures participated in this study. All participating persons gave their consent to the study following protocol which was approved by the ethical committee. Individuals with structural deformities that could hinder accurate anthropological assessment were excluded from the study.
Measurement of digits length
The lengths of the second (2D), third (3D), fourth (4D), and fifth (5D) fingers of both hands were measured with an electronic Vernier calliper (precision 0.01 mm, Neiko Corporation, USA). ^{[2]} The length of each finger was defined as the distance from the ventral proximal crease of the digit to the tip. If there was a band of creases at the base of the digit, measurements were made from the most proximal of these. Measurements were performed twice. Finger lengths were calculated as the mean values of the first and second measurement of the length of each digit.
Blood specimen collection and processing
Blood specimens (2 ml each) were also taken into plain tubes by venepuncture. Serum testosterone levels were measured by enzyme immunoassay using a commercially available testosterone EIA test kit (BioCheck Inc., Foster City, CA).
Data analyses
Data were expressed as mean ± standard deviation. The Student's ttest was used to test the difference in the mean of all variables between boys and girls from the Hausa and Igbo ethnic groups. Pearson's correlation coefficient was used to test the relationship between testosterone and all the measured anthropometric parameters. P < 0.05 was deemed statistically significant and SPSS version 16 (IBM Corp., New York) was used for the statistical analyses.
Results   
The mean digit lengths of all digits of the right and left hand in both sexes with the characteristics of their values (minimum and maximum) are represented in [Table 1]. The mean values of all the finger digits are lower in females than in males. All parameters are statistically significant at P < 0.001. The digit ratios of the right and left hands in both sexes are presented in [Table 2]. Females had higher 2D: 4D (0.99 ± 0.01) than males (0.98 ± 0.01) in both hands. This difference was statistically significant at P < 0.001. The result also revealed statistical significant difference (P < 0.001) in other digit ratios except 3D:5D and 3D:4D. Difference in digit ratio between the right and left fingers was observed in 3D:5D in both sexes, however, this difference was not statistically significant. The highest difference in digit ratios between sexes was observed in 2D:5D ratios of both hands, with P values lower than 0.001. [Table 3] demonstrated that in males, serum testosterone had an inverse relationship with 2D: 4D in both hands, while in females it correlated inversely with 2D:4D of the left hand only. These relationships were statistically significant at P < 0.05 and P < 0.01.  Table 1: Descriptive statistics showing the finger length of hands in both sexes
Click here to view 
 Table 2: Descriptive statistics showing the digit ratios of the hands in both sexes
Click here to view 
 Table 3: Correlation matrix of serum testosterone level and anthropometric variables in female (black font) and male (red font) participants
Click here to view 
Discussion   
Digit ratio (2D:4D) is a sexually dimorphic biometric marker that is determined genetically by the Hox genes. ^{[9],[11]} 2D:4D is under the influence of prenatal oestrogen and testosterone levels in utero. ^{[11],[17]} The 2D:4D ratio in the sample of the Igbo population that was studied was found to be significantly higher in women than in men, a finding that is consistent with other studies of 2D:4D in other ethnic groups in Nigeria and other countries. ^{[8],[9],[10],[18]} In our study we found that the mean 2D:4D ratio was 0.98 ± 0.01 in males and 0.99 ± 0.01 in females, these values are higher than reported in previous studies carried out in Europe (the mean 2D:4D ratio in males and females were found respectively to be 0.98 and 1.00 in U.K., 0.95 and 0.98 in Austria, 0.93 and 0.94, in Greece, 0.93 and 0.95, in Finland, 0.96 and 0.97 in USA, 0.95 and 0.97 in Canada. ^{[9],[15]}
In the Zulu and Sardinian ethnic groups, 2D:4D (0.95) and (0.98) respectively were found to be equal in men and women. ^{[9]} This did not present sexual dimorphism and thus are contrary to the findings in our study and other studies on other ethnic groups, in which the 2D:4D ratio was found to be sexually dimorphic. ^{[15],[18],[19]} Digit ratio is a biometric marker that turns to become very useful for the prevention and prognosis of diseases that are dependent on sex hormones. ^{[2],[7]}
Although many studies on 2D:4D have been carried out during the last decade, studies on the other digit ratios are very limited. ^{[9],[20],[21]} In our study we found that the other digit ratios are also sexually dimorphic: They are lower in men than women. The difference in digits ratio between sexes was statistically significant for all digit ratios of both hands except for the 3D:5D and 3D:4D ratio of the both hands.
The study of 2D:4D and other digit ratios, as well as their variation among population groups and among individuals may serve as a useful tool for establishing their values as biomarkers. ^{[9],[20],[21]} 2D:4D and other digit ratios appear to be attractive anthropometric traits that probably deserve attention by anthropologists, scientists, and clinicians since these digit ratios have continued to show promise as biomarkers of health and diseases. ^{[7],[22],[23]}
Our results support the sexual dimorphism of digit ratios but not the lateralized character of digit ratios found in other studies. ^{[13],[15],[24],[25]} Our study found an equal digit ratio in both hands except in 3D:5D in both sexes and 2D:5D in females (right = 1.27 ± 0.08 and left = 1.26 ± 0.07). The differences in the results among population groups could be attributed to different digit pattern expression, which may reflect differences in the influence of genetic or environmental parameters. ^{[26]} Previous studies have shown that testosterone is related negatively to 2D:4D whereas estrogen correlates positively with 2D:4D. ^{[11],[17],[27]} Lutchmaya et al.^{[28]} also showed a relationship between 2D:4D measured in children and testosterone concentration measured in samples of amniotic fluid and cord blood collected during their gestation. ^{[11],[23]} This work showed a significant negative relationship between 2D:4D and serum testosterone, further establishing the organizational effect of testosterone on digit ratio.
Conclusion   
In conclusion, the result of this study has shown that the 2D:4D digit ratio is sexually dimorphic and it is a biometric marker that can be useful in the prognosis and prevention of diseases that are dependent on sex hormones, thus deserving further probing in different directions for possible discovery of associations with other traits by scientists and clinicians.
References   
1.  Phelps VR. Relative index finger length as a sexinfluenced trait in man. Am J Hum Genet 1952;4:7289. 
2.  Manning JT, Scutt D, Wilson J, LewisJones DI. The ratio of 2 ^{nd} to 4 ^{th} digit length: A predictor of sperm numbers and concentrations of testosterone, luteinizing hormone and oestrogen. J Hum Reprod 1998;13:30004. 
3.  Manning JT, Callow M, Bundred PE. Finger and toe ratios in humans and mice: Implications for the aetiology of diseases influenced by HOX genes. Med Hypotheses 2002;60:3403. 
4.  Peters M, Mackenzie K, Bryden P. Finger length and distal finger extent patterns in humans. AmJ Phys Anthropol 2002;117:20917. 
5.  Galis F, Ten Broek CM, Van Dongen S, Wijnaendts LC. Sexual dimorphism in the prenatal digit ratio (2D:4D). Arch Sex Behav 2010;39:5762. 
6.  Berenbaum SA, Bryk KK, Nowak N, Quigley CA, Moffat S. Fingers as a marker of prenatal androgen exposure. Endocrinology 2009;150:511924. 
7.  Oyeyemi BF, Iyiola OA, Oyeyemi AW, Oricha KA, Anifowoshe AT, Alamukii NA. Sexual dimorphism in ratio of second and fourth digits and its relationship with metabolic syndrome indices and cardiovascular risk factors. J Res Med Sci 2014;19:2349. [ PUBMED] 
8.  Danborno AB, Adebisi SS, Adelaiye AB, Ojo SA. Relationship between second and fourth digit lengths, digit ratio (2D:4D) and aggression in Nigerians. J Environ Neurosci Biomed 2008;2:22934. 
9.  Kyriakidis I, Papaioannidou P. Epidemiologic study of the sexually dimorphic second to fourth digit ratio (2D:4D) and other finger ratios in Greek population Coll Antropol 2008;32:10938. 
10.  Gwunireama IU, Ihemelandu, EC. Geographical influence on digit ratio (2D:4D): A case study of Andoni and Ikwerre ethnic groups in Niger delta, Nigeria. J Appl Biosci 2010;27:173641. 
11.  Zheng Z, Cohn MJ. Developmental basis of sexually dimorphic digit ratios. Proc Natl Acad Sci U S A 2011;108:1628994. 
12.  Fink B, Neave N, Manning JT. Second to fourth digit ratio, body mass index, waisttohip ratio, and waisttochest ratio: Their relationships in heterosexual men and women. Ann Hum Biol 2003;30:72838. 
13.  Danborno B, Adebisi SS, Adelaiye AB, Ojo SA. Relationship between digit ratio (2D:4D) and birth weight in Nigerians. Anthropol 2010;12:12730. 
14.  Wilson GD. Fingerlength as an index of assertiveness in women. Pers Individ Dif 1983;4:1112. 
15.  Manning JT, Fink B, Trivers R. Digit ratio (2D:4D) and gender inequalities across nations. Evol Psychol 2014;12:75768. 
16.  Brown WM, Hines M, Fane BA, Breedlove SM. Masculinized finger length patterns in human males and females with congenital adrenal hyperplasia. Horm Behav 2002;42:3806. 
17.  Putz DA, Gaulin SJ, Sporter RJ, McBurney DH. Sex hormones and finger length: What does 2D:4D indicate? Evol Hum Behav 2004;25:18299. 
18.  Oladipo G, Fawehinmi H, Edibamode E, Osunwoke E, Ordu KS. Second to fourth digit ratio in Nigerian Igbos and Yorubas. Sci Res Essay 2009;4:11468. 
19.  Ibegbu AO, Danjuma ZC, Hamman WO, Umana UE, Ikyembe D, Musa SA. Association of the index (2 ^{nd} ) and ring (4 ^{th} ) digit ratios with some physical attributes in Ebira Ethnic Group of Nigeria. Appl Technol Innov 2012;7:4654. 
20.  Ghosh D, Garth D. A study of finger length relation (ring finger & little finger, i.e. 4D:5D) with Human personality. Psychiatry online Journal 2005;3:19. 
21.  Eachus P. Finger length, digit ratio and gender differences in sensation seeking and internet selfefficacy. Issues Inform Sci Inform Technol 2007,4:691701. 
22.  Manning JT. Digit Ratio: A Pointer to Fertility, Behavior, and Health. New Brunswick, NJ: Rutgers University Press; 2002. 
23.  Mitsui T, Araki A, Imai A, Sato S, Miyashita C, Ito S, et al. Effects of prenatal leydig cell function on the ratio of the second to fourth digit lengths in schoolaged children. PLoS One 2015;10:e0120636. 
24.  Longman D, Stock JT, Wells JC. Digit ratio (2D:4D) and rowing ergometer performance in males and females. Am J Phys Anthropol 2011;144:33741. 
25.  Mitsui T, Araki A, Imai A, Sato S, Miyashita C, Ito S, et al. Effects of prenatal leydig cell function on the ratio of the second to fourth digit lengths in schoolaged children. PLoS One 2015;10:e0120636. 
26.  Auger J, Le Denmat D, Berges R, Doridot L, Salmon B, CanivencLavier MC, et al. Environmental levels of oestrogenic and antiandrogenic compounds feminize digit ratios in male rats and their unexposed male progeny. Proc Biol Sci 2013;280:20131532. 
27.  Breedlove SM. Minireview: Organizational hypothesis: Instances of the fingerpost. Endocrinology 2010;151:411622. 
28.  Lutchmaya S, BaronCohen S, Raggatt P, Knickmeyer R, Manning JT. 2 ^{nd} to 4 ^{th} digit ratios, fetal testosterone and estradiol. Early Hum Dev 2004;77:238. 
[Table 1], [Table 2], [Table 3]
