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ORIGINAL ARTICLE
Year : 2015  |  Volume : 3  |  Issue : 1  |  Page : 29-32

Histomorphologic effects of Cannabis sativa on the brains of adult Wistar rats


Department of Human Anatomy and Cell Biology, College of Health Sciences, Delta State University, Abraka, Nigeria

Correspondence Address:
Emmanuel Igho Odokuma
Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, College of Health Sciences, Delta State University, PMB - 1, Abraka
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2315-7992.160747

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Introduction: Cannabis sativa is one of the species of cannabis plant. The objective of this study was to determine the short- and long-term effects of Cannabis sativa on the brains of adult Wistar rats. Materials and Methods: Thirty (30) healthy adult Wistar rats of both sexes were used as experimental animals. The Cannabis sativa leaves obtained were extracted at the Laboratory Facility of the Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta State University, Abraka, Nigeria. The animals were grouped as follows: Positive control (one: Group I), negative control (one: Group II), and experimental groups (three: Groups III-V); different doses of the drug in volume were calculated (0.10 mL for acute study, 0.23 mL for subacute study, and 0.50 mL for subchronic study) and administered orally with the use of an improvised orogastric cannula. The animals were sacrificed and the organs harvested for manual tissue processing and hematoxylin-eosin (H&E) staining. The stained slides were interpreted with the aid of a light microscope and analyzed for histomorphological changes across the groups. Results: The results showed that the histomorphologic changes induced by Cannabis sativa in short- and long-term studies caused extensive cerebral gliosis in the brains of adult Wistar rats. Conclusion: It could, therefore, be deduced that there were both dose- and time-dependent toxic effects of Cannabis sativa on the experimental animals. Cannabis sativa was shown to cause marked neuronal changes in the brains of adult Wistar rats.


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